Summary
In previous studies we have shown that glycolysis is strongly inhibited in cultured calvaria cells treated with ethane-1-hydroxy-1, 1-diphosphonate (EHDP) or dichloromethanediphosphonate (Cl2MDP). This study shows that the energy charge of the adenylate pool, and the ATP and Pi content, were not changed by treatment with diphosphonates except for a slight decrease of ATP and Pi at 0.25 mM Cl2 MDP. The uptake of Pi was diminished by 50% and 20% in cells treated for 6 days with Cl2MDP or EHDP, respectively, but not when diphosphonates were present only during the uptake studies.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature
M.D. Francis, R.G.G. Russell and H. Fleisch, Science165, 1264 (1969).
H. Fleisch, R.G.G. Russell and M.D. Francis, Science165, 1262 (1969).
O.L.M. Bijvoet, A.J.G. Nollen, T.J.J.H. Slooff and R. Feith, Acta orthop. scand.45, 926 (1974).
S.L. Stover, H.R. Hahn and J.M. Miller, Paraplegia14, 146 (1976).
R.D. Altman and F. Singer, eds. Proceedings of the Kroc Foundation Conference on Paget's Disease of Bone. Arthritis Rheumatism23, 1155 (1980).
F.J.M. van Breukelen, O.L.M. Bijvoet and A.T. van Oosterom, Lancet1, 803 (1979).
E.S. Siris, W.H. Sherman, D.C. Baquiran, J.P. Schlatterer, E.F. Osserman and R.E. Canfield, New Engl. J. Med.302, 310 (1980).
D.K. Fast, R. Felix, C. Dowse, W.F. Neuman and H. Fleisch, Biochem. J.172, 97 (1978).
R. Felix, D.K. Fast, J.D. Sallis and H. Fleisch, Calcif. Tissue Int.30, 163 (1980).
R. Felix and H. Fleisch, Biochem. J.183, 73 (1979).
R. Felix and H. Fleisch, Biochem. J.196, 237 (1981).
H.L. Guenther, H.E. Guenther and H. Fleisch, Biochem. J.196, 303 (1981).
H.L. Guenther, H.E. Guenther and H. Fleisch, Biochem. J.184, 203 (1979).
D.E. Atkinson, Biochemistry7, 4030 (1968).
S. Addanki, J.F. Sotos and P.D. Rearick, Analyt. Biochem.14, 261 (1966).
Y. Sugino and Y. Miyoshi, J. biol. Chem.239, 2360 (1964).
H.J. Altmann, E. Fürstenau, A. Gielewski and L. Scholz, Z. analyt. Chem.256, 274 (1971).
G.A. Norman, M.J. Follett and D.A. Hector, J. Chromat.90, 105 (1974).
J.P. Schwartz and G.S. Johnson, Archs Biochem. Biophys.173, 237 (1976).
J.J. Ende and H.J.M. van Rooijen, Proc. K. ned. Akad. Wet.C-82, 43 (1979).
J.J. Ende and H.J.M. van Rooijen, Proc. K. ned. Akad. Wet.C-82, 55 (1979).
R. Wu, J. biol. Chem.234, 2806 (1959).
U. Tröhler, J.-P. Bonjour and H. Fleisch, Kidney Int.8, 6 (1975).
Author information
Authors and Affiliations
Additional information
Acknowledgments. We thank Miss B. Wyss for her skilled technical assistance. This work has been supported by the Swiss National Science Foundation (grant Nos 3.725.76 and 3.824.79), by the Procter & Gamble Company, USA, and by the Ausbildungs- und Förderungsfonds der Arbeitsgemeinschaft für Osteosynthese (AO), Chur, Switzerland.
Rights and permissions
About this article
Cite this article
Felix, R., Fleisch, H. Effect of diphosphonates on ATP and Pi content, Pi uptake and energy charge of cultured calvaria cells. Experientia 38, 644–646 (1982). https://doi.org/10.1007/BF01964068
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01964068