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Alteration in thermal stability of ribosomes fromDrosophila melanogaster with age

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Summary

Thermal analysis of high salt (0.5 M) washed ribosomal monomers from young and old maleDrosophila melanogaster revealed an 8°C downshift in the mean temperature of denaturation (Tm). Moreover, there was observed a marked loss in the ability of ribosomes extracted from older flies to reassociate upon cooling. These observations suggest that age-dependent alterations in the structural integrity of the rRNA-r protein complex could, at least in part, be responsible for the diminished capacity for protein synthesis in this species with advancing age.

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Authors and Affiliations

  1. Department of Biological Sciences, Drexel University, 19104, Philadelphia, Pa., USA

    G. T. Baker, R. E. Zschunke & E. M. Podgorski Jr

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  1. G. T. Baker
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  2. R. E. Zschunke
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  3. E. M. Podgorski Jr
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Additional information

Acknowledgments. We thank Dr R. Christian for critical reading of the manuscript and Ms P.C. Snelling for her skillful preparation of the manuscript. This work was supported by Drexel Research Scholar Award to G.T.B. and the Paul Glenn Foundation for Medical Research.

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Baker, G.T., Zschunke, R.E. & Podgorski, E.M. Alteration in thermal stability of ribosomes fromDrosophila melanogaster with age. Experientia 35, 1053–1054 (1979). https://doi.org/10.1007/BF01949936

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