Summary
The terminals of the fast axon on extensor tibiae muscle fibres of Locusta were examined in untreated nerve-muscle preparations and in preparations stimulated electrically at frequencies varying from 0.5 to 100 Hz. The ultrastructure of the terminals in preparations stimulated at the lower range of these frequencies, which induce twitch contractions of the muscles, is similar to that of the controls. Stimulation at the higher frequencies induced tetanic muscle responses and rapid fatigue of the muscles after which they would not respond again to high frequency stimulation for about 1 h. This loss and recovery of the responses of the muscles is correlated with changes in the ultrastructural appearance of the terminals, in particular in the number and shape of the synaptic vesicles. The ultrastructure of these “recovering” axon terminals closely resembles that of the controls.
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Bennett, M.V.L., Model, P.G., Highstein, S.M.: Stimulation-induced depletion of vesicles, fatigue of transmission and recovery processes at a vertebrate central synapse. In: Cold Spring Harbor Symposium on quantitative biology. The Synapse XL pp. 25–36. New York: Cold Spring Harbor Laboratory 1976
Botham, R.P.: An examination of the ultrastructure of the insect neuromuscular junction and investigation of the neurotransmitter. Ph. D. Thesis. C.N.A.A., London (1977)
Botham, R.P., Beadle, D.J., Hart, R.J., Potter, C., Wilson, R.G.: Changes in the distribution, size and shape of synaptic vesicles in neuromuscular junctions of Locusta migratoria L. after stimulation and rest. Cell Tissue Res. 203, 373–378 (1979)
Ceccarelli, B., Hurlbut, W.P., Mauro, A.: Turnover of transmitter and synaptic vesicles at the frog neuromuscular junction. J. Cell Biol. 57, 499–524 (1973)
Clements, A.N., May, T.E.: Studies on locust neuromuscular physiology in relation to glutamic acid. J. Exp. Biol. 60, 673–705 (1974)
Hoyle, G.: Neural control of skeletal muscle. In: The Physiology of Insecta 4 (M. Rockstein, ed.), pp. 176–232. New York: Academic Press 1974
Hubbard, J.I., Kwanbunbumpen, S.: Evidence for the vesicle hypothesis. J. Physiol. 194, 407–420 (1968)
McKinlay, R.G., Usherwood, P.N.R.: The role of the synaptic vesicles in transmission at the insect nerve-muscle junction. Life Sci. 13, 1051–1056 (1973)
Reynolds, E.S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell. Biol. 17, 208–212 (1963)
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R.P. Botham gratefully acknowledges the SRC for financial assistance
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Botham, R.P., Beadle, D.J., Hart, R.J. et al. Effects of stimulation and rest on the ultrastructure of the excitatory neuromuscular junctions of Locusta migratoria L.. Cell Tissue Res. 203, 367–371 (1979). https://doi.org/10.1007/BF00233265
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DOI: https://doi.org/10.1007/BF00233265