biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 59:485-490, 2015 | DOI: 10.1007/s10535-015-0520-3

Application of internal transcribed spacers and maturase K markers for identifying Anoectochilus, Ludisia, and Ludochilus

J. -R. Chen1, Y. -J. Shiau2,*
1 Biotechnology Division, Agricultural Research Institute, Wufeng, Taichung, Taiwan, ROC
2 Plant Germplasm Division, Agricultural Research Institute, Wufeng, Taichung, Taiwan, ROC

Internal transcribed spacer (ITS) regions and maturase K (matK) sequence polymorphisms provide an efficient tool for discrimination and conservation of genetic resources of Anoectochilus species. The objectives of this study were to develop markers specifically distinguishing A. formosanus Hayata from closely related A. koshunensis Hayata, A. roxburghii (Wall.) Lindl., and Ludisia discolor (Ker Gawl.) A. Rich. and to identify a molecular phylogenetic relationship of a new intergeneric BC1F1 hybrid - Ludochilus Jin-Chai. Specific primers for nuclear ITS regions and chloroplast matK sequences were designed and converted into cleaved amplified polymorphic sequence (CAPS) markers. Results show that the matK sequences obtained corresponded to pseudogenes and that their digestion with enzyme HinfI revealed a polymorphic pattern in A. formosanus and A. koshunensis. The pedigree of Lud. Jin-Chai, which was derived from the cross between Lus. discolor and A. formosanus, was also confirmed based on ITS and matK CAPS markers.

Keywords: chloroplast matK sequence; intergeneric hybrid; jewel orchids; plant phylogenetic analysis
Subjects: internal transcribed spacer; maturase K; chloroplast genes; intergeneric hybrid; CAPS markers

Received: March 18, 2013; Revised: February 9, 2015; Accepted: February 18, 2015; Published: September 1, 2015  Show citation

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Chen, J.-R., & Shiau, Y.-J. (2015). Application of internal transcribed spacers and maturase K markers for identifying Anoectochilus, Ludisia, and Ludochilus. Biologia plantarum, 59(3), 485-490. doi: 10.1007/s10535-015-0520-3
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