biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 48:481-489, 2004 | DOI: 10.1023/B:BIOP.0000047141.49470.77

Differences in Anatomical Structure and Lignin Content of Roots of pedunculate Oak and Wild Cherry-Tree Plantlets During Acclimation

A. Soukup1, J. Malá2, M. Hrubcová3, J. Kálal2, O. Votrubová1, M. Cvikrová3
1 Department of Plant Physiology, Faculty of Science, Charles University, Prague 2, Czech Republic
2 Forestry and Game Management Research Institute, Zbraslav-Strnady, Czech Republic
3 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 6, Czech Republic

The lignin contents and anatomical structure of roots of wild cherry (Prunus avium L.) and pedunculate oak (Quercus robur L.) plantlets were compared to explain differences in response during transfer from in vitro to ex vitro conditions. Lignification of cell walls increased significantly in both oak and cherry roots during the period of acclimation and finally lignin content of root tissues of in vitro propagated plantlets reached the levels not significantly different from seedlings grown in soil. Later on when secondary tissues appeared, lignified secondary xylem constituted most of the tissues of both species. The most conspicuous interspecific difference in root structure was the presence of phi-thickenings in cortical layers just outer to endodermis in cherry roots cultivated ex vitro. Formation of phi-thickenings was avoided in vitro and their presence thus seems to be under environmental control. Suberised well established exodermis was present in roots of oak but not detected in those of cherry. Very early development of exodermis in oak roots, preceding suberisation of endodermis, was recorded in vitro but not in well aerated soil. While multilayered and well-developed cork occurred in oak, only thin walled and less suberised secondary dermal tissues were found in cherry.

Keywords: exodermis; in vitro; lignin; phi-thickenings; Prunus avium; Quercus robur; root development
Subjects: auxins; Casparian bands; endodermis, exodermis; exodermis, endodermis; in vitro culture, acclimation, adaptation; in vitro culture, nutrient media modification; in vitro culture, root, rooting; irradiation, continuous, anatomical structure, root; lignin, root development; nutrient medium, Murashige and Skoog; nutrient medium, woody plant medium, WPM (Loyd and McCown); pedunculate oak, root development; phi (Φ) thickening; Prunus avium; Quercus robur; root development, lignin; suberisation; wild cherry, root development; xylem, root lignification

Published: December 1, 2004  Show citation

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Soukup, A., Malá, J., Hrubcová, M., Kálal, J., Votrubová, O., & Cvikrová, M. (2004). Differences in Anatomical Structure and Lignin Content of Roots of pedunculate Oak and Wild Cherry-Tree Plantlets During Acclimation. Biologia plantarum, 48(4), 481-489. doi: 10.1023/B:BIOP.0000047141.49470.77
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