biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 39:169-177, 1997 | DOI: 10.1023/A:1000372315936

Stimulation of H+transport activity of vacuolar H+ATPase by activation of H+PPase in Kalanchoë blossfeldiana

E. Fischer-Schliebs1, J.-B. Mariaux2, U. Lüttge1
1 Institut für Botanik, Technische Hochschule Darmstadt, Darmstadt, Germany
2 Max Planck Institut für Züchtungsforschung, Köln, Germany

In Kalanchoë blossfeldiana cv. Tom Thumb the initial rate of ATP-dependent H+-transport into tonoplast vesicles was stimulated up to three times if the H+-ATPase (EC 3.6.1.3) was energized a few minutes after pre-energization of the H+-PPase (EC 3.6.1.1). H+-PPase-activated ATP-dependent H+-transport was observed in plants of K. blossfeldiana cultivated in short day (SD) or long day (LD) conditions expressing different degrees of crassulacean acid metabolism (CAM). However, based on the higher activity and protein amount of H+-PPase and H+-ATPase present in the vacuolar membrane of SD plants the maximum H+-transport activity in the stimulated mode of the H+-ATPase was significantly higher in tonoplast vesicles of SD plants than of LD plants. Hence, a co-ordinated action of the H+-PPase and H+-ATPase at the tonoplast of Kalanchoë could allow a higher transport capacity at the vacuolar membrane when plants perform high CAM. Immunoprecipitation experiments with an antiserum raised against the A-subunit of the vacuolar H+-ATPase of Mesembryanthemum crystallinum L. showed that in SD and LD plants of K. blossfeldiana the H+-PPase was co-precipitated with the vacuolar H+-ATPase holoenzyme. The co-percipitation of the two transport proteins indicates a close structural localization of the H+-PPase and the A-subunit of the vacuolar H+-ATPase.

Keywords: Crassulacean acid metabolism; photoperiodism; proton transport; tonoplast

Prepublished online: March 1, 1997; Published: September 1, 1997  Show citation

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Fischer-Schliebs, E., Mariaux, J.-B., & Lüttge, U. (1997). Stimulation of H+transport activity of vacuolar H+ATPase by activation of H+PPase in Kalanchoë blossfeldiana. Biologia plantarum, 39(2), 169-177. doi: 10.1023/A:1000372315936
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