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Antimitotic activity of EA21b mammary-carcinoma extract

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Abstract

Many tumors produce factors that affect cell-cycle and cell proliferation. In the present study we have analyzed the effect of a mammary-tumor extract injection on the mitotic activity of several organs in young male C3H/S mice previously standardized for circadian periodicity. One-half of the animals received an intraperitoneal EA21b tumor extract dose at 16:00 h, while the other half received saline. Animals were sacrificed on the following day at 08:00, 12:00 or 16:00 h. 4 h after receiving an injection of colchicine by the same route. Samples of duodenum, kidney, liver, and submaxillary gland were excised and processed for hematoxylin-eosin staining. Mitotic indices, expressed as the number of colchicine-arrested metaphases per 1,000 nuclei, were assessed in convoluted tubule epithelium, duodenal crypt enterocytes, hepatocytes and submaxillary gland ductal and acinar sialocytes. All values were expressed as mean ± SEM. Statistical analyses were performed by ANOVA, Bonferroni and Student’s t-tests. In contrast to the mitotic indices reductions observed in renal convoluted tubules cells and duodenal crypt enterocytes, neither the submaxillary gland nor the liver were found to contain cell types whose mitotic activity was affected by the tumor extract. We conclude that EA21b mammary carcinoma contains one or more factors that inhibit the proliferation of selected populations of normal cells.

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Authors and Affiliations

  1. Cátedra de Histología y Embriología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 60 y 118, CP 1900, La Plata, Buenos Aires, Argentina

    Mirta Alicia Flamini, Norma Viviana González & Claudio Gustavo Barbeito

  2. Instituto de Patología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina

    Mirta Alicia Flamini & Claudio Gustavo Barbeito

  3. Cátedra de Histología y Embriología Animal, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina

    Norma Viviana González

  4. CONICET, National Scientific Research Council, Argentina

    Claudio Gustavo Barbeito

Authors
  1. Mirta Alicia Flamini
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  2. Norma Viviana González
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  3. Claudio Gustavo Barbeito
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Correspondence to Claudio Gustavo Barbeito.

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Flamini, M.A., González, N.V. & Barbeito, C.G. Antimitotic activity of EA21b mammary-carcinoma extract. Biologia 62, 238–242 (2007). https://doi.org/10.2478/s11756-007-0039-y

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  • DOI: https://doi.org/10.2478/s11756-007-0039-y

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