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Assignment of the Gene for Porcine Insulin-Like Growth Factor Binding Protein 1 to Chromosome 18 and Detection of Polymorphisms in Intron 2 by PCR–RFLP

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Abstract

We have obtained a partial cDNA and three BAC clones for the porcine insulin-like growth factor binding protein 1 gene (IGFBP-1). Results of fluorescence in situ and radiation hybrid (RH) mapping assigned this gene to porcine chromosome (SSC) 18q24-qter. We found two types of polymerase chain reaction–restriction-fragment-length polymorphisms (PCR–RFLP) in intron 2 by using FokI and AluI.

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Correspondence to Noriyuki Hamasima.

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Inage-Miyake, Y., Shimanuki, Si., Itoh, T. et al. Assignment of the Gene for Porcine Insulin-Like Growth Factor Binding Protein 1 to Chromosome 18 and Detection of Polymorphisms in Intron 2 by PCR–RFLP. Biochem Genet 43, 79–85 (2005). https://doi.org/10.1007/s10528-005-1069-1

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  • DOI: https://doi.org/10.1007/s10528-005-1069-1

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