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Quantitative Bestimmung von Sterigmatocystin in verschimmelten pflanzlichen Nahrungsmitteln

Quantitative determination of sterigmatocystin in mouldy vegetable foods

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Summary

The quantitative determination of sterigmatocystin is achieved by two-dimensional thin-layer chromatography (TLC) of extracts from vegetable foods and measurement of the fluorescence of the spots on AlCl3-treated TLC plates. Samples and controls (references) are applied to the TLC plates in a way that permits their quick identification and assay. Acetonitrile-KCl solution has proved to be the most advantageous extracting agent. Losses of less than 5 % were observed in tests with added toxin.

Zusammenfassung

Ein Verfahren für die quantitative Bestimmung von Sterigmatocystin in pflanzlichen Nahrungsmitteln durch zweidimensionale DC-Trennung eines Extraktes und Fluorescenzintensitätsmessung der mit AlCl3 behandelten DC-Platte wird beschrieben. Durch die hier vorgeschlagene Auftrageweise auf der DC-Platte ist ein rasches Identifizieren und Auswerten von Probe- und Eichflecken möglich. Als Extraktionsmittel hat sich Acetonitril-KCl-Lösung am besten bewährt. Versuche mit zugesetztem Toxin zeigten Verluste von weniger als 5 %.

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Authors and Affiliations

  1. Institut für Biochemie der Johannes-Gutenberg-Universität, Joh.-Joachim-Becher-Weg 28, D-6500, Mainz

    R. Schmidt, K. Neunhoeffer & K. Dose

Authors
  1. R. Schmidt
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  2. K. Neunhoeffer
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  3. K. Dose
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Schmidt, R., Neunhoeffer, K. & Dose, K. Quantitative Bestimmung von Sterigmatocystin in verschimmelten pflanzlichen Nahrungsmitteln. Z. Anal. Chem. 299, 382–384 (1979). https://doi.org/10.1007/BF00704972

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  • DOI: https://doi.org/10.1007/BF00704972

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