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HPLC-Analytik hochpolarer Pyridoxyl-Alkaloide im Säugerblut

HPLC-analysis of highly polar pyridoxyl alkaloids in mammalian blood

Endogene Alkaloide im Menschen. Teil 6

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  • Biochemische und Klinische analyse
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Summary

A HPLC-method for the determination of pyridoxyl alkaloids — endogenous condensation products of vitamin B6 and biogenic amines, resp. amino acids — is presented. On the basis of ion-pair assisted reversed-phase HPLC, conditions were found for the first time that allow an efficient separation and detection of these — partially extremely polar — heterocycles even from complex biofluids like mammalian blood and urine. The method allows to investigate the condensation kinetics in vitro, and — in combination with radiochemical methods — to prove the formation of pyridoxyl alkaloids in vivo. In single cases, the analysis is hampered by other constituents of blood or urine; the detection limit (S/N = 2) for a 250 μl test volume under optimum conditions is 1–2 ng.

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Author notes

  1. G. Bringmann

    Present address: Institut für Organische Chemie der Universität Würzburg, Am Hubland, D-8700, Würzburg, Bundesrepublik Deutschland

  2. S. Schneider

    Present address: Department of Chemistry, University of Wisconsin, 53706, Madison, WI, USA

Authors and Affiliations

  1. Organisch-Chemisches Institut der Universität Münster, Orléansring 23, D-4400, Münster, Bundesrepublik Deutschland

    G. Bringmann & S. Schneider

  2. Zentrale Tierexperimentelle Einrichtung der Medizinischen Fakultät, Universität Münster, Domagkstrasse 15a, D-4400, Münster, Bundesrepublik Deutschland

    K. D. Richter

Authors
  1. G. Bringmann
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  2. S. Schneider
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  3. K. D. Richter
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Additional information

Diese Arbeit ist Herrn Professor Dr. Helmut Dörfel, BASF AG, Ludwigshafen, zum 60. Geburstag gewidmet.

Teil 5: Bringmann G, Hille A, Zsiška M (1987) Heterocycles, im Druck

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Bringmann, G., Schneider, S. & Richter, K.D. HPLC-Analytik hochpolarer Pyridoxyl-Alkaloide im Säugerblut. Z. Anal. Chem. 329, 480–484 (1987). https://doi.org/10.1007/BF00480092

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  • DOI: https://doi.org/10.1007/BF00480092

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