A new kinetic photometric method for the simultaneous determination of metals based on catalytic and activating effects

Summary

The potential use of the activating effect of Pb(II) on the Mn(II)-catalysed oxidation of Tiron by hydrogen peroxide in the presence of 1,10-phenanthroline for the simultaneous determination of the two metals was investigated. The results obtained allowed the development of a new kinetic photometric method for the simultaneous determination of Pb(II) and Mn(II). The catalysed reaction was monitored by the initial rate method, which was applied to absorbance-time curves. Different Mn(II) concentrations were used to construct calibration graphs by plotting the slopes of the photometric curves obtained against the Mn(II) concentration at each Pb(II) concentration assayed. A new calibration graph was obtained in terms of the Pb(II) concentration from the slopes of such graphs. By applying the standard-addition method to the sample to be assayed a third graph was obtained, the slope and intercept of which provided the analytical concentration of Pb(II) and Mn(II), respectively. The optimized values of the different variables involved were used to determine Mn(II) and Pb(II) over the concentration ranges 1–5 and 200–800 ng/ml, respectively.