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Certification of fat-soluble vitamins, carotenoids, and cholesterol in human serum: Standard reference material 968b

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Abstract

In Standard Reference Material 968b, fat-soluble vitamins and cholesterol in human serum, certified values are provided for cholesterol, retinol, retinyl palmitate, α-tocopherol, trans-β-carotene, total β-carotene (trans plus cis isomers), total α-carotene, and lutein. Non-certified values are also reported for γ-tocopherol (includes β-tocopherol), δ-tocopherol, zeaxanthin, β-cryptoxanthin, trans-lycopene, trans-lycopene, trans-α-carotene, total lycopene, 9-cis-β-carotene, 13-plus 15-cis-β-carotene, and 15-cis-β-carotene. Both certified and non-certified values are based on the agreement among results from three different liquid Chromatographic analytical procedures developed at NIST and from an interlaboratory comparison exercise among institutions that participate in a NIST-managed Micronutrients Measurement Quality Assurance Program. Cholesterol is certified in this material using the NIST isotope dilution/mass spectrometric definitive method.

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Authors and Affiliations

  1. Analytical Chemistry Division, NIST, 20899, Gaithersburg, MD, USA

    Jeanice Brown Thomas, Polly M. Ellerbe, Lorna T. Sniegoski, David L. Duewer & Katherine E. Sharpless

  2. Biotechnology Division, NIST, 20899, Gaithersburg, MD, USA

    Margaret C. Kline

  3. Statistical Engineering Division, NIST, 20899, Gaithersburg, MD, USA

    Susannah B. Schiller

Authors
  1. Jeanice Brown Thomas
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  2. Margaret C. Kline
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  3. Susannah B. Schiller
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  4. Polly M. Ellerbe
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  5. Lorna T. Sniegoski
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  6. David L. Duewer
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  7. Katherine E. Sharpless
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Thomas, J.B., Kline, M.C., Schiller, S.B. et al. Certification of fat-soluble vitamins, carotenoids, and cholesterol in human serum: Standard reference material 968b. Fresenius J Anal Chem 356, 1–9 (1996). https://doi.org/10.1007/s0021663560001

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  • DOI: https://doi.org/10.1007/s0021663560001

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