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Reagentless amperometric biosensor highly sensitive to hydrogen peroxide based on the incorporation of Meldola Blue, fumed-silica and horseradish peroxidase into carbon paste

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Abstract

A reagentless amperometric sensor highly sensitive to H2O2 has been prepared by incorporating fumed silica, horseradish peroxidase (HRP) and Meldola Blue into carbon paste. The efficient mediating ability to shift electrons between HRP and the carbon paste electrode via Meldola Blue was investigated by cyclic voltammetric and amperometric measurements. Reproducibility, response time, detection limit, selectivity and effects of applied potential, temperature and pH on the response of the sensor are reported. The high sensitivity of the sensor with a detection limit of 0.1 μmol/1 arose from the high efficiency of the bioelectrocatalytic reduction of hydrogen peroxide via HRP and Meldola Blue. The dependence of the Michaelis-Menten constant on the applied potential and the mediator concentration has been investigated and the results are presented.

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Authors and Affiliations

  1. Department of Chemistry and Chemical Engineering, Shanghai University, 200072, Shanghai, People’s Republic of China

    Haiying Liu, Xiaolin Zhang, Jianjun Wei, Zunan Qiu, Hongbin Li, Xinxin Wu & Deyao Qi

  2. Department of Environmental Protection, Suzhou Institute of City Construction and Environmental Protection, Suzhou, 300111, Jiansu Province, People’s Republic of China

    Zhanen Zhang

  3. Department of Chemistry, Fudan University, 200433, Shanghai, People’s Republic of China

    Yubo Fan, Miao Dai & Jiaqi Deng

Authors
  1. Haiying Liu
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  2. Zhanen Zhang
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  3. Yubo Fan
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  4. Miao Dai
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  5. Xiaolin Zhang
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  6. Jianjun Wei
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  7. Zunan Qiu
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  8. Hongbin Li
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  9. Xinxin Wu
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  10. Jiaqi Deng
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  11. Deyao Qi
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Liu, H., Zhang, Z., Fan, Y. et al. Reagentless amperometric biosensor highly sensitive to hydrogen peroxide based on the incorporation of Meldola Blue, fumed-silica and horseradish peroxidase into carbon paste. Z. Anal. Chem. 357, 297–301 (1997). https://doi.org/10.1007/s002160050156

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  • DOI: https://doi.org/10.1007/s002160050156

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