Summary
Investigations on the effects of pH, temperature, type and concentration of respiration substrates and oxygen tension on the reduction rate of derivatives of 1-phenylazo-2-naphthol and of a variety of textile dyes served as a basis for establishing a bioassay for strictly reproducible measurements of the microbial reduction rate of azo dyes. Standard organism was a strain of Bacillus cereus isolated from soil. Dye reduction occurred with the standard organism and other facultatively or obligatory aerobic bacteria in exclusively anoxic conditions. In principle, first order kinetics of decolorization were found. Reduction products may however inhibit the reaction. All dyes not measurably reduced by living cells of B. cereus were decolorized by cell extracts of the same species. Dyes adsorbed by the cell walls were in most cases reduced at slow rates and did not influence the simultaneous reduction of non-adsorbable dyes in the medium. The observations confirm the hypothesis advanced by Gingell and Walker (1971) of an intracellular, non-enzymatic reduction of azo compounds by reduced flavin nucleotides. The rate of permeation of the dyes through the cell membrane is the primordial ratelimiting step in the microbial decolorization of azo dyes. Sulfonic acid substitution seems to be an effective inhibitor of permeation.
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References
Chung KT, Fulk GE, Egan M (1978) Appl Environ Microbiol 35: 558–562
Dubin P, Wright KL (1975) Xenobiotica 5: 563–571
Gingell R, Walker R, (1971) Xenobiotica 1, 231–239
Horitsu H, Takada M, Idaka E, Tomoyeda M, Ogawa T (1977) Eur J Appl Microbiol 4: 217–224
Idaka E, Ogawa T, Horitsu H, Tomoyeda M (1978) JSDC (March) pp. 91–94
Kalkar HM (1947) J Biol Chem 167: 461 In: Bergmeyer HU (ed) 2nd edn. Verlag Chemie, Weinheim, pp 139
Leidner H, Gloor R, Wüest D, Wuhrmann K (1979) Xenobiotica (in press)
Mecke R Jr, Schmähl D (1957) Arzneim Forsch 7: 335–340
Meyer U, Overney G, Wattenwyl A v (1979) Textilveredlung 14: 15–20
Overney G (1979) Diss ETH Zürich no 6421
Roxon JJ, Ryan AJ, Wright SE (1966) Food Cosmet Toxicol 4: 419–426
Roxon JJ, Ryan AJ, Wright SE (1967) Food Cosmet Toxicol 5: 645–656
Urushigawa Y, Yonezawa Y (1977) Bull Environ Contamin Toxicol 17: 214–218
Walker R (1970) Review section. Food Cosmet Toxicol 8: 659–675
Walker R, Gingell R, Murrells DF (1971) Xenobiotica 1: 221–229
Walker R, Ryan AJ (1972) Some molecular parameters influencing rate of reduction of azo compounds by intestinal microflora. In: Bridges JW, Parke DV (eds) Biological oxidation of nitrogen in organic molecules. Taylor and Francis Ltd, London pp 171–174
Wuhrmann K (1957) Schweiz Z Hydrol 19: 409–427
Wuhrmann K (1964) Schweiz Z Hydrol 26: 520–558
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Wuhrmann, K., Mechsner, K. & Kappeler, T. Investigation on rate — Determining factors in the microbial reduction of azo dyes. European J. Appl. Microbiol. Biotechnol. 9, 325–338 (1980). https://doi.org/10.1007/BF00508109
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DOI: https://doi.org/10.1007/BF00508109