Abstract
Cell lines derived from the rat suprachiasmatic nucleus (SCN) were screened for circadian clock properties distinctive of the SCN in situ. Immortalized SCN cells generated robust rhythms in uptake of the metabolic marker 2-deoxyglucose and in their content of neurotrophins. The phase relationship between these rhythms in vitro was identical to that exhibited by the SCN in vivo. Transplantation of SCN cell lines, but not mesencephalic or fibroblast lines, restored the circadian activity rhythm in arrhythmic, SCN-lesioned rats. Thus, distinctive oscillator, pacemaker, and clock properties of the SCN are not only retained but also maintained in an appropriate circadian phase relationship by immortalized SCN progenitors.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Biological Clocks*
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Brain-Derived Neurotrophic Factor / metabolism
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Cell Line
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Cell Transplantation
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Circadian Rhythm*
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Deoxyglucose / metabolism
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Glucose-6-Phosphate / analogs & derivatives
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Glucose-6-Phosphate / metabolism
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Glycogen / metabolism
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Graft Survival
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Male
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Motor Activity
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Nerve Growth Factors / metabolism
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Neurons / metabolism
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Neurons / physiology*
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Neurons / transplantation
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Neurotrophin 3
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Rats
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Rats, Sprague-Dawley
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Stem Cells / cytology
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Stem Cells / metabolism
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Stem Cells / physiology*
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Suprachiasmatic Nucleus / cytology*
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Suprachiasmatic Nucleus / metabolism
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Suprachiasmatic Nucleus / physiology
Substances
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Brain-Derived Neurotrophic Factor
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Nerve Growth Factors
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Neurotrophin 3
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2-deoxyglucose-6-phosphate
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Glucose-6-Phosphate
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Glycogen
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Deoxyglucose