Abstract
The plant hormone abscisic acid (ABA) regulates potassium and chloride ion channels at the plasma membrane of guard cells, leading to stomatal closure that reduces transpirational water loss from the leaf. The tobacco Nt-SYR1 gene encodes a syntaxin that is associated with the plasma membrane. Syntaxins and related SNARE proteins aid intracellular vesicle trafficking, fusion, and secretion. Disrupting Nt-Syr1 function by cleavage with Clostridium botulinum type C toxin or competition with a soluble fragment of Nt-Syr1 prevents potassium and chloride ion channel response to ABA in guard cells and implicates Nt-Syr1 in an ABA-signaling cascade.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Abscisic Acid / pharmacology*
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Amino Acid Sequence
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Animals
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Botulinum Toxins / metabolism
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Cell Membrane / physiology
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Chloride Channels / physiology*
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Genes, Plant
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Genetic Complementation Test
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Ion Channel Gating / drug effects
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Membrane Proteins / chemistry
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Membrane Proteins / genetics
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Membrane Proteins / physiology*
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Molecular Sequence Data
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Nicotiana / genetics
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Nicotiana / physiology*
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Oocytes
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Patch-Clamp Techniques
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Plant Growth Regulators / pharmacology*
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Plant Leaves / physiology*
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Plants, Toxic*
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Potassium Channels / physiology*
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Qa-SNARE Proteins
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Signal Transduction
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Xenopus
Substances
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Chloride Channels
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Membrane Proteins
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Plant Growth Regulators
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Potassium Channels
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Qa-SNARE Proteins
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Abscisic Acid
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Botulinum Toxins
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botulinum toxin type C
Associated data
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GENBANK/AF112863
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GENBANK/AF112864