Abstract
Early events in the humoral immune response were visualized in lymph nodes by simultaneous tracking of antigen-specific CD4 T and B cells after immunization. The T cells were initially activated in the T cell areas when the B cells were still randomly dispersed in the B cell-rich follicles. Both populations then migrated to the edges of the follicles and interacted there, resulting in CD154-dependent B cell proliferation and germinal center formation. These results provide visual documentation of cognate T-B cell interactions and localize them to the follicular border.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adoptive Transfer
-
Animals
-
Antibody Formation*
-
Antigen Presentation
-
B-Lymphocytes / cytology
-
B-Lymphocytes / immunology*
-
CD4-Positive T-Lymphocytes / cytology
-
CD4-Positive T-Lymphocytes / immunology*
-
CD40 Ligand
-
Cell Movement
-
Dendritic Cells / immunology
-
Germinal Center / immunology
-
Immunization
-
Immunoglobulin M / analysis
-
Lymph Nodes / cytology
-
Lymph Nodes / immunology*
-
Lymphocyte Activation*
-
Membrane Glycoproteins / immunology
-
Mice
-
Mice, Inbred BALB C
-
Mice, Inbred C57BL
-
Mice, Transgenic
-
Plasma Cells / immunology
Substances
-
Immunoglobulin M
-
Membrane Glycoproteins
-
CD40 Ligand