Abstract
Candidate mammalian odorant receptors were first cloned some 6 years ago. The physiological function of these receptors in initiating transduction in olfactory receptor neurons remains to be established. Here, a recombinant adenovirus was used to drive expression of a particular receptor gene in an increased number of sensory neurons in the rat olfactory epithelium. Electrophysiological recording showed that increased expression of a single gene led to greater sensitivity to a small subset of odorants.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviridae / genetics
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Adenoviridae / physiology
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Aldehydes / metabolism
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Aldehydes / pharmacology*
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Animals
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Electrophysiology
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Female
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Gene Expression
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Genetic Vectors
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Green Fluorescent Proteins
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Luminescent Proteins / analysis
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Luminescent Proteins / genetics
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Male
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Odorants*
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Olfactory Receptor Neurons / physiology*
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Olfactory Receptor Neurons / virology
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Patch-Clamp Techniques
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Rats
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Rats, Sprague-Dawley
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Receptors, Odorant / genetics
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Receptors, Odorant / metabolism
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Receptors, Odorant / physiology*
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Recombinant Proteins
Substances
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Aldehydes
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Luminescent Proteins
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Receptors, Odorant
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Recombinant Proteins
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odorant receptor 17
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Green Fluorescent Proteins
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caprylic aldehyde