Abstract
Heterotrimeric guanosine triphosphate (GTP)-binding proteins (G proteins) may participate in membrane traffic events. A complementary DNA (cDNA) was isolated from a mouse pituitary cDNA library that corresponded to an alternatively spliced form of the gene encoding the G protein alpha subunit G alpha i2. The cDNA was identical to that encoding G alpha i2 except that the region encoding for the carboxyl-terminal 24 amino acids was replaced by a longer region encoding 35 amino acids that have no sequence similarity with G alpha i2 or other members of the G protein family. This alternative spliced product and the corresponding protein (sGi2) were present in several tissues. Specific antibodies revealed that sGi2 was localized in the Golgi apparatus, suggesting a role in membrane transport. Thus, alternative splicing may generate from a single gene two G protein alpha subunits with differential cellular localization and function.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing*
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Line
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Cell Membrane / metabolism
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Coatomer Protein
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DNA, Complementary / genetics
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GTP-Binding Protein alpha Subunit, Gi2
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GTP-Binding Protein alpha Subunits, Gi-Go*
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GTP-Binding Proteins / analysis
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GTP-Binding Proteins / chemistry
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GTP-Binding Proteins / genetics
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GTP-Binding Proteins / metabolism*
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Golgi Apparatus / chemistry
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Golgi Apparatus / metabolism*
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Mice
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Microtubule-Associated Proteins / analysis
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Molecular Sequence Data
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Oncogene Proteins / analysis
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Oncogene Proteins / chemistry
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Oncogene Proteins / genetics
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Oncogene Proteins / metabolism*
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Proto-Oncogene Proteins*
Substances
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Coatomer Protein
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DNA, Complementary
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Microtubule-Associated Proteins
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Oncogene Proteins
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Proto-Oncogene Proteins
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GTP-Binding Proteins
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GTP-Binding Protein alpha Subunit, Gi2
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GTP-Binding Protein alpha Subunits, Gi-Go
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Gnai2 protein, mouse