Abstract
The T cell antigen receptor (TCR) beta chain regulates early T cell development in the absence of the TCR alpha chain. The developmentally controlled gene described here encodes the pre-TCR alpha (pT alpha) chain, which covalently associates with TCR beta and with the CD3 proteins forms a pre-TCR complex that transduces signals in immature thymocytes. Unlike the lambda 5 pre-B cell receptor protein, the pT alpha chain is a type I transmembrane protein whose cytoplasmic tail contains two potential phosphorylation sites and a Src homology 3 (SH3)-domain binding sequence. Pre-TCR alpha transfection experiments indicated that surface expression of the pre-TCR is controlled by additional developmentally regulated proteins. Identification of the pT alpha gene represents an essential step in the structure-function analysis of the pre-TCR complex.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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CD3 Complex / metabolism
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Cell Line
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Cloning, Molecular*
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DNA, Complementary / genetics
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Gene Expression Regulation, Developmental*
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Gene Rearrangement
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Membrane Glycoproteins / chemistry
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Membrane Glycoproteins / genetics*
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Membrane Glycoproteins / metabolism
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Mice
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Mice, Inbred C57BL
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Molecular Sequence Data
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Open Reading Frames
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Phosphorylation
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Polymerase Chain Reaction
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Rabbits
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Receptors, Antigen, T-Cell, alpha-beta / chemistry
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Receptors, Antigen, T-Cell, alpha-beta / genetics*
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Receptors, Antigen, T-Cell, alpha-beta / metabolism
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Signal Transduction
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T-Lymphocytes / immunology*
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Transfection
Substances
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CD3 Complex
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DNA, Complementary
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Membrane Glycoproteins
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Receptors, Antigen, T-Cell, alpha-beta
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pre-T cell receptor alpha
Associated data
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GENBANK/U16958
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GENBANK/U27268