Abstract
The rate at which the TATA-binding protein (TBP) interacts with the TATA element and promotes transcription by RNA polymerase II was determined in yeast cells. A TBP derivative with altered TATA-element specificity was rapidly induced, and transcription from promoters with appropriately mutated TATA elements was measured. Without a functional activator protein, basal transcription was observed only after a lag of several hours. In contrast, GCN4-activated transcription occurred rapidly upon induction of the TBP derivative. These results suggest that accessibility of TBP to the chromatin template in vivo is rate limiting and that activation domains increase recruitment of TBP to the promoter.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Binding Sites
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Chromatin / metabolism
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Copper / pharmacology
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DNA-Binding Proteins / metabolism*
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Fungal Proteins / metabolism
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Fungal Proteins / pharmacology
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Hydro-Lyases / genetics
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Molecular Sequence Data
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Protein Kinases / metabolism
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Protein Kinases / pharmacology
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Saccharomyces cerevisiae Proteins*
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TATA Box*
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TATA-Box Binding Protein
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Templates, Genetic
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Transcription Factors / metabolism*
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Transcription Factors / pharmacology
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Transcriptional Activation*
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Yeasts / genetics
Substances
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Chromatin
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DNA-Binding Proteins
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Fungal Proteins
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Saccharomyces cerevisiae Proteins
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TATA-Box Binding Protein
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Transcription Factors
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Copper
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Protein Kinases
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Hydro-Lyases
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imidazoleglycerolphosphate dehydratase