Abstract
The antigen of a monoclonal antibody that is specific for cells of human carcinoma of the colon is a monosialoganglioside as determined by the direct binding of antibody to thin-layer chromatograms of total lipid extracts of tissues. Binding of antibody to chromatograms is detected by autoradiography after the application of iodine-125-labeled F(ab')2 of rabbit immunoglobulin G antibodies to mouse immunoglobulins.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenocarcinoma / immunology*
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Antibodies, Neoplasm / immunology*
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Antibody Specificity
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Antigens, Neoplasm / immunology*
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Antigens, Neoplasm / isolation & purification
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Cell Line
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Chromatography, Thin Layer
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Colonic Neoplasms / immunology*
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Gangliosides / immunology*
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Gangliosides / isolation & purification
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Humans
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Melanoma / immunology
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Neuraminidase / pharmacology
Substances
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Antibodies, Neoplasm
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Antigens, Neoplasm
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Gangliosides
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colon carcinoma monosialoganglioside antigen
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Neuraminidase