Abstract
Irradiation Lucifer yellow-filled neurons with intense blue light in the presence of 3,3'-diaminobenzidine produces an electron-opaque osmiophilic polymer within the injected cells. This technique is valuable when cobalt or horseradish peroxidase injections are difficult or when a second intracellular marker is needed to demonstrate neuronal contacts.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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3,3'-Diaminobenzidine
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Animals
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Astacoidea
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Ganglia / physiology
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Ganglia / ultrastructure
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In Vitro Techniques
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Isoquinolines*
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Leeches
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Light
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Microscopy, Electron / methods
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Neurons / physiology
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Neurons / ultrastructure*
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Synapses / ultrastructure
Substances
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Isoquinolines
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3,3'-Diaminobenzidine
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lucifer yellow