Abstract
Amiloride inhibited the ouabain-sensitive rate of oxygen consumption (QO2) of a suspension of rabbit intact proximal tubules in the presence of different concentrations of extracellular sodium. Measurements of the ouabain-sensitive QO2 in the presence of nystatin, the tissue sodium and potassium contents of the tubules in suspension, and the sodium- and potassium-dependent adenosinetriphosphatase (Na,K-ATPase) activity of lysed tubule membranes indicated that the effect of amiloride was due to a direct inhibition of the Na,K-ATPase activity of the proximal tubule.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amiloride / pharmacology*
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Animals
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Dose-Response Relationship, Drug
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Female
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Ion Channels / drug effects
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Kidney Tubules, Proximal / drug effects
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Kidney Tubules, Proximal / enzymology*
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Nystatin / pharmacology
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Ouabain / pharmacology
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Oxygen Consumption / drug effects
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Pyrazines / pharmacology*
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Rabbits
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Rats
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Sodium / metabolism
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Sodium-Potassium-Exchanging ATPase / antagonists & inhibitors*
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Sodium-Potassium-Exchanging ATPase / metabolism
Substances
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Ion Channels
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Pyrazines
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Nystatin
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Ouabain
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Amiloride
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Sodium
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Sodium-Potassium-Exchanging ATPase