Abstract
Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amidohydrolases / metabolism
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Animals
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Antibodies, Monoclonal / biosynthesis
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Antibodies, Monoclonal / physiology*
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Antibody Specificity
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Antigens / immunology
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Catalysis*
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Chemical Phenomena
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Chemistry
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Hemocyanins / analogs & derivatives
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Hemocyanins / immunology
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Hydrolysis
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Immunization
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Kinetics
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Mice
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Organophosphorus Compounds / immunology
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Substrate Specificity
Substances
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Antibodies, Monoclonal
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Antigens
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Organophosphorus Compounds
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keyhole limpet hemocyanin phosphonamidate conjugate
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Hemocyanins
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Amidohydrolases
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keyhole-limpet hemocyanin