The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein

Science. 1985 Dec 20;230(4732):1344-9. doi: 10.1126/science.2933810.

Abstract

A component present in splicing extracts selectively binds the 3' splice site of a precursor messenger RNA (pre-mRNA) transcript of a human beta-globin gene. Since this component can be immunoprecipitated by either autoantibodies of the Sm class or antibodies specifically directed against trimethylguanosine, it is a small nuclear ribonucleoprotein (snRNP). Its interaction with the 3' splice site occurs rapidly even at 0 degrees C, does not require adenosine triphosphate, and is altered by certain mutations in the 3' splice site region. Binding is surprisingly insensitive to treatment of the extract with micrococcal nuclease. The U5 particle is the only abundant Sm snRNP with a capped 5' end that is equally resistant to micrococcal nuclease. This suggests that, in addition to the U1 and U2 snRNP's, U5 snRNP's participate in pre-mRNA splicing.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Binding Sites
  • Globins / genetics
  • Humans
  • Nucleic Acid Conformation
  • Nucleic Acid Precursors / genetics*
  • Nucleic Acid Precursors / metabolism
  • Protein Binding
  • RNA Precursors
  • RNA Splicing*
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism
  • Ribonucleoproteins / genetics*
  • Ribonucleoproteins / metabolism
  • Ribonucleoproteins, Small Nuclear
  • Transcription, Genetic

Substances

  • Nucleic Acid Precursors
  • RNA Precursors
  • RNA, Messenger
  • Ribonucleoproteins
  • Ribonucleoproteins, Small Nuclear
  • Globins

Associated data

  • GENBANK/J00093
  • GENBANK/J00094
  • GENBANK/J00096
  • GENBANK/J00158
  • GENBANK/J00159
  • GENBANK/J00160
  • GENBANK/J00161
  • GENBANK/J00162
  • GENBANK/J00163
  • GENBANK/J00164
  • GENBANK/J00165
  • GENBANK/J00166
  • GENBANK/J00167
  • GENBANK/J00168
  • GENBANK/J00169
  • GENBANK/J00170
  • GENBANK/J00171
  • GENBANK/J00172
  • GENBANK/J00173
  • GENBANK/J00174
  • GENBANK/J00175
  • GENBANK/J00177
  • GENBANK/J00178
  • GENBANK/J00179
  • GENBANK/K01239
  • GENBANK/K01890
  • GENBANK/K02544
  • GENBANK/M18047
  • GENBANK/M19067
  • GENBANK/X00423