Catalytic antibodies with lipase activity and R or S substrate selectivity

Science. 1989 Apr 28;244(4903):437-40. doi: 10.1126/science.2717936.

Abstract

The specific hydrolysis of unactivated esters bearing an R or S enantiomeric alcohol has been achieved by two separate classes of catalytic antibodies induced to bind either the R or S substrates. The antibodies exhibit rate accelerations (10(3) to 10(5] above background hydrolysis that, coupled with their antipodal specificity, provide a novel set of reagents for use in synthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal* / immunology
  • Antibody Specificity
  • Antigens / immunology
  • Benzyl Alcohols / metabolism
  • Catalysis*
  • Esters / metabolism
  • Haptens
  • Hemocyanins / immunology
  • Hydrolysis
  • Immunization
  • Kinetics
  • Lipase / metabolism*
  • Mice
  • Mice, Inbred A
  • Molecular Structure
  • Organophosphonates / immunology
  • Stereoisomerism
  • Substrate Specificity

Substances

  • Antibodies, Monoclonal
  • Antigens
  • Benzyl Alcohols
  • Esters
  • Haptens
  • Organophosphonates
  • Hemocyanins
  • methylphenyl carbinol
  • Lipase
  • keyhole-limpet hemocyanin