Abstract
Mouse 3T3 cell lines capable of constitutively synthesizing an RNA complementary to the messenger RNA encoding TIMP, tissue inhibitor of metalloproteinases, were constructed by transfection with appropriate plasmid constructs. Many of the lines were down-modulated for TIMP messenger RNA levels and secreted less TIMP into the culture medium. In comparison to noninvasive, nontumorigenic controls, these cells not only were invasive in a human amnion invasion assay, but also were tumorigenic and metastatic in athymic mice. These results indicate that TIMP suppresses oncogenicity, at least in immortal murine 3T3 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line
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Cell Transformation, Neoplastic*
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Cells, Cultured
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Enzyme Inhibitors / genetics*
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Enzyme Inhibitors / metabolism
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Female
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Metalloendopeptidases / antagonists & inhibitors
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Mice
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Mice, Nude
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Neoplasm Metastasis
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Pituitary Neoplasms / genetics
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Pituitary Neoplasms / pathology
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RNA / genetics*
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RNA, Antisense
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RNA, Messenger / antagonists & inhibitors*
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RNA, Messenger / genetics
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Tissue Inhibitor of Metalloproteinases
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Transfection
Substances
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Enzyme Inhibitors
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RNA, Antisense
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RNA, Messenger
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Tissue Inhibitor of Metalloproteinases
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RNA
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Metalloendopeptidases