Abstract
Cytotoxic T lymphocytes (CTLs) recognize class I major histocompatibility complex (MHC) molecules associated with antigenic peptides derived from endogenously synthesized proteins. Binding to such peptides is a requirement for class I assembly in the endoplasmic reticulum (ER). A mutant human cell line, T2, assembles and transports to its surface some, but not all, class I MHC molecules. The class I molecules expressed on the surface of T2 do not present peptides derived from cytosolic antigens, although they can present exogenously added peptides to CTL. The transported class I molecules may interact weakly with an unknown retaining factor in the ER such that they can assemble despite the relative shortage of peptides.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigen-Presenting Cells / immunology*
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Antigens / immunology
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Antigens, Viral / immunology
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B-Lymphocytes / immunology
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Capsid / immunology
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Cell Line
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Endoplasmic Reticulum / immunology
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Gene Expression
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H-2 Antigens / genetics
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H-2 Antigens / immunology
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HLA Antigens / genetics
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Histocompatibility Antigens Class I / immunology*
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Histocompatibility Antigens Class II / genetics
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Humans
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Mice
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Mutation
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Ovalbumin / immunology
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Peptides / immunology
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T-Lymphocytes, Cytotoxic / immunology
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Transfection
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Tumor Cells, Cultured
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Viral Core Proteins / immunology
Substances
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Antigens
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Antigens, Viral
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H-2 Antigens
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HLA Antigens
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Histocompatibility Antigens Class I
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Histocompatibility Antigens Class II
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Peptides
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Viral Core Proteins
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Ovalbumin