Abstract
Microfluorometric measurements of cerebral cortical mitochondrial respiration in vivo are obscured by hemodynamic and oximetric artifacts. Isosbestic fluorometry provides appropriate correction for these vascular phenomena and permits simultaneous evaluation of mitochondrial nicotinamide adenine dinucleotide redox state, microcirculatory volume, and hemoglobin oxygenation.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Brain Chemistry*
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Cerebral Cortex / analysis*
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Dogs
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Fluorometry / instrumentation
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Fluorometry / methods*
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Hemoglobins / analysis
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Hypoxia, Brain / metabolism
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Mitochondria / metabolism
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NAD / analysis*
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NAD / metabolism
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Oxidation-Reduction
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Oxygen / blood
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Rhodamines
Substances
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Hemoglobins
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Rhodamines
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NAD
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Oxygen