Abstract
Herpes simplex virus type 1 (HSV-1) is a ubiquitous pathogen responsible for considerable morbidity in the general population. The results presented herein establish the basic fibroblast growth factor (FGF) receptor as a means of entry of HSV-1 into vertebrate cells. Inhibitors of basic FGF binding to its receptor and competitive polypeptide antagonists of basic FGF prevented HSV-1 uptake. Chinese hamster ovary (CHO) cells that do not express FGF receptors are resistant to HSV-1 entry; however, HSV-1 uptake is dramatically increased in CHO cells transfected with a complementary DNA encoding a basic FGF receptor. The distribution of this integral membrane protein in vivo may explain the tissue and cell tropism of HSV-1.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adsorption
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Amino Acid Sequence
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Animals
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Binding, Competitive
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Cell Line
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Cell Membrane / microbiology
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Cricetinae
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DNA / genetics
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Fibroblast Growth Factors / antagonists & inhibitors
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Fibroblast Growth Factors / metabolism
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Fibroblast Growth Factors / pharmacology
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Heparitin Sulfate / metabolism
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Molecular Sequence Data
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Peptide Fragments / pharmacology
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / physiology*
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Receptors, Fibroblast Growth Factor
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Simplexvirus / physiology*
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Transfection
Substances
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Peptide Fragments
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Receptors, Cell Surface
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Receptors, Fibroblast Growth Factor
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Fibroblast Growth Factors
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DNA
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Heparitin Sulfate