Abstract
With [3H]cytidine as a precursor, phosphoinositide turnover can be localized in brain slices by selective autoradiography of the product [3H]cytidine diphosphate diacylglycerol, which is membrane-bound. In the cerebellum, glutamatergic stimulation elicits an increase of phosphoinositide turnover only in Purkinje cells and the molecular layer. In the hippocampus, both glutamatergic and muscarinic cholinergic stimulation increase phosphoinositide turnover, but with distinct localizations. Cholinergic stimulation affects CA1, CA3, CA4, and subiculum, whereas glutamatergic effects are restricted to the subiculum and CA3. Imaging phosphoinositide turnover in brain slices, which are amenable to electrophysiologic studies, will permit a dynamic localized analysis of regulation of this second messenger in response to synaptic stimulation of specific neuronal pathways.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alanine / analogs & derivatives
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Alanine / pharmacology
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Animals
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Autoradiography
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Brain / metabolism*
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Carbachol / pharmacology
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Cerebellum / drug effects
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Cerebellum / metabolism
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Cycloleucine / analogs & derivatives
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Cycloleucine / pharmacology
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Cytidine / metabolism
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Cytidine Diphosphate Diglycerides / metabolism
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Glutamates / physiology
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Glutamic Acid
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Hippocampus / drug effects
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Hippocampus / metabolism
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Neomycin / pharmacology
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Phosphatidylinositols / metabolism*
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Pirenzepine / pharmacology
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Purkinje Cells / metabolism
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Rats
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Receptors, Muscarinic / drug effects
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Receptors, Muscarinic / physiology
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Tissue Distribution
Substances
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Cytidine Diphosphate Diglycerides
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Glutamates
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Phosphatidylinositols
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Receptors, Muscarinic
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2-amino-3-phosphonopropionic acid
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Cycloleucine
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1-amino-1,3-dicarboxycyclopentane
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Pirenzepine
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Glutamic Acid
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Cytidine
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Carbachol
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Neomycin
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Alanine