DNA hydrolyzing autoantibodies

Science. 1992 May 1;256(5057):665-7. doi: 10.1126/science.1585181.

Abstract

A DNA-nicking activity was detected in the sera of patients with various autoimmune pathologies and was shown to be a property of autoantibodies. The DNA hydrolyzing activity, which was purified by affinity and high-performance liquid chromatography, corresponded in size to immunoglobulin M (IgM) and IgG and had a positive response to antibodies to human IgG. The DNA hydrolyzing autoantibodies were stable to acid shock and yielded a DNA degradation pattern that was different from that of deoxyribonuclease (DNase) I and blood DNase.

Publication types

  • Comparative Study

MeSH terms

  • Acetates / pharmacology
  • Acetic Acid
  • Autoantibodies / isolation & purification
  • Autoantibodies / metabolism*
  • Autoimmune Diseases / metabolism*
  • Chromatography, High Pressure Liquid
  • DNA / metabolism*
  • DNA Polymerase I / metabolism
  • Deoxyribonuclease I / metabolism
  • Electrophoresis, Agar Gel
  • Humans
  • Immunoglobulin G / isolation & purification
  • Immunoglobulin G / metabolism
  • Immunoglobulin M / isolation & purification
  • Immunoglobulin M / metabolism
  • Kinetics
  • Lupus Erythematosus, Systemic / immunology
  • Plasmids

Substances

  • Acetates
  • Autoantibodies
  • Immunoglobulin G
  • Immunoglobulin M
  • DNA
  • DNA Polymerase I
  • Deoxyribonuclease I
  • Acetic Acid