Salmonella is a common foodborne pathogen that can cause food poisoning, posing a serious threat to human health. Therefore, quickly, sensitively, and accurately detecting
Salmonella is crucial to ensuring food safety. For the
Salmonella hilA gene, we designed Recombinase-aided amplification (RAA) primers
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Salmonella is a common foodborne pathogen that can cause food poisoning, posing a serious threat to human health. Therefore, quickly, sensitively, and accurately detecting
Salmonella is crucial to ensuring food safety. For the
Salmonella hilA gene, we designed Recombinase-aided amplification (RAA) primers and dsDNA-specific nuclease (DNase) probes. The ideal primer and probe combination was found when conditions were optimized. Under UV light, a visual
Salmonella detection technique (RAA-dsDNase) was developed. Additionally, the RAA-dsDNase was modified to further reduce pollution hazards and simplify operations. One-pot RAA-dsDNase-UV or one-pot RAA-dsDNase-LFD was developed as a
Salmonella detection method, using UV or a lateral flow dipstick (LFD) for result observation. Among them, one-pot RAA-dsDNase and one-pot RAA-dsDNase-LFD had detection times of 50 min and 60 min, respectively, for detecting
Salmonella genomic DNA. One-pot RAA-dsDNase-UV had a detection limit of 10
1 copies/μL and 10
1 CFU/mL, while one-pot RAA-dsDNase-LFD had a sensitivity of 10
2 copies/μL and 10
2 CFU/mL. One-pot RAA-dsDNase-UV and one-pot RAA-dsDNase-LFD assays may identify 17 specific
Salmonella serovars witho ut causing a cross-reaction with the remaining 8 bacteria, which include
E. coli. Furthermore,
Salmonella in tissue and milk samples has been reliably detected using both approaches. Overall, the detection method developed in this study can quickly, sensitively, and accurately detect
Salmonella, and it is expected to become an important detection tool for the prevention and control of
Salmonella in the future.
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