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Selective enrichment and sensitive detection of candidate disease biomarker using a novel surfactant-coated magnetic nanoparticles

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, , Citation R Y Capangpangan et al 2014 IOP Conf. Ser.: Mater. Sci. Eng. 64 012022 DOI 10.1088/1757-899X/64/1/012022

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1 Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan

2 Molecular Science and Technology, Taiwan International Graduate Program, Taiwan

3 Department of Chemistry, National Taiwan University, Taipei, Taiwan

4 Nanoscience and Technology Program, Taiwan International Graduate Program, Taiwan

5 Institute of Chemistry, Academia Sinica, Taipei, Taiwan

6 En Hou Polymer Chemical Ind. Co., Ltd., Taiwan

7 Department of Material Science and Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan

8 contributed equally to this work

9 To whom any correspondence should be addressed

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1757-899X/64/1/012022

Abstract

In this study, novel surfactant-coated magnetic nanoparticles were synthesized and evaluated for enrichment performance towards the sensitive detection of disease biomarkers. Surfactants with phosphate ester groups (RD35A and RD66) were used as a coating to reduce aggregation and to enhance the nanoparticle dispersion. Importantly, sensitive enrichment of the target proteins using the antibody-functionalized magnetic nanoparticles (Ab@MNP) was obtained, with a five-fold increase in recovery compared to uncoated magnetic nanoparticles. Similarly, phosphopeptide enrichment using the NTA@MNP in standard samples showed that the nanoparticles could selectively enrich phosphorylated peptides.

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10.1088/1757-899X/64/1/012022