Citation:

fsx017

Name: Seawater carbonate chemistry and fertilization success of red abalone
Published: 2017-08-30
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Haliotis rufescens; Laboratory experiment; Mollusca; North Pacific; Oxygen; Reproduction; Single species; Temperate; Temperature

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Abstract:

Acidification, deoxygenation, and warming are escalating changes in coastal waters throughout the world ocean, with potentially severe consequences for marine life and ocean-based economies. To examine the influence of these oceanographic changes on a key biological process, we measured the effects of current and expected future conditions in the California Current Large Marine Ecosystem on the fertilization success of the red abalone (Haliotis rufescens). Laboratory experiments were used to assess abalone fertilization success during simultaneous exposure to various levels of seawater pH (gradient from 7.95 to 7.2), dissolved oxygen (DO) (60 and 180 µm/kg SW) and temperature (9, 13, and 18 °C). Fertilization success declined continuously with decreasing pH but dropped precipitously below a threshold near pH 7.55 in cool (9 °C upwelling) to average (13 °C) seawater temperatures. Variation in DO had a negligible effect on fertilization. In contrast, warmer waters (18 °C) often associated with El Nino Southern Oscillation conditions in central California acted antagonistically with decreasing pH, largely reducing the strong negative influence below the pH threshold. Experimental approaches that examine the interactive effects of multiple environmental drivers and also strive to characterize the functional response of organisms along gradients in environmental change are becoming increasingly important in advancing our understanding of the real-world consequences of changing ocean conditions.

Keyword(s):

Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Haliotis rufescens; Laboratory experiment; Mollusca; North Pacific; Oxygen; Reproduction; Single species; Temperate; Temperature

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2017-08-30.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Boch, Charles A		study
2	Species	Species		Boch, Charles A
3	Registration number of species	Reg spec no		Boch, Charles A
4	Uniform resource locator/link to reference	URL ref		Boch, Charles A		WoRMS Aphia ID
5	Date	Date		Boch, Charles A		abalone acquired from aquaculture farm and placed into brooding tanks
6	Date	Date		Boch, Charles A		abalone spawned for experiment
7	Experiment	Exp		Boch, Charles A		unique designation for a particular experiment
8	Individuals	Ind	#	Boch, Charles A		number of females induced for egg collection pooled
9	Individuals	Ind	#	Boch, Charles A		number of males induced for sperm collectionpooled
10	Individuals	Ind	#	Boch, Charles A		number of males that actually spawned sperm
11	Individuals	Ind	#	Boch, Charles A		number of females that actually spawned eggs
12	Name	Name		Boch, Charles A		Microscope used for checking fertilization success
13	Date	Date		Boch, Charles A		fixed samples were processed
14	Name	Name		Boch, Charles A		Name of person who processed fixed samples
15	Sample ID	Sample ID		Boch, Charles A		unique identifier of fixed sample relating to a unique syringe number
16	Treatment	Treat		Boch, Charles A
17	Temperature, water	Temp	°C	Boch, Charles A		predicted
18	Salinity	Sal		Boch, Charles A
19	Alkalinity, total	AT	µmol/kg	Boch, Charles A
20	Alkalinity, total, standard deviation	AT std dev	±	Boch, Charles A
21	pH	pH		Boch, Charles A		total scale, predicted
22	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Boch, Charles A		predicted
23	Oxygen	O2	µmol/l	Boch, Charles A		dissolved oxygen, predicted
24	Carbon, inorganic, dissolved	DIC	µmol/kg	Boch, Charles A		predicted
25	pH	pH		Boch, Charles A	Potentiometric	total scale, measured
26	pH	pH		Boch, Charles A		temperature adjusted pH of the Sentron ISFET estimates for each sample
27	Oxygen	O2	µmol/l	Boch, Charles A		dissolved oxygen, measured
28	Time in seconds	Time	s	Boch, Charles A		total amount of time (in seconds) that eggs and sperm were allowed to interact in gas-tight syringe
29	Time in hours	Time	h	Boch, Charles A		total amount of time (in hours) after sperm washing the fertilized eggs were allowed to incubate
30	Time in minutes	Time	min	Boch, Charles A		total amount of time (in minutes) after sperm washing the fertilized eggs were allowed to incubate
31	Eggs, unfertilized	Unfert eggs	#	Boch, Charles A		the number of eggs that were not noticeably fertilized in fixed samples--ie, not fertilized if less than 2 cells
32	Eggs, two-cell stage	Eggs 2-cell	#	Boch, Charles A		total number of cells that were at the 2 cell stage in fixed samples
33	Eggs, four-cell stage	Eggs 4-cell	#	Boch, Charles A		total number of cells that were at the 4 cell stage in fixed samples
34	Eggs	Eggs	#	Boch, Charles A		total number of cells that were at the 4 plus cell stage in fixed samples; greater than 4 cells becomes subjective due to hidden surfaces
35	Eggs	Eggs	#	Boch, Charles A		number of damaged cells
36	Eggs, abnormal	Abn eggs	#	Boch, Charles A		number cells abnormal in appearance; normal development is described in literature
37	Comment	Comment		Boch, Charles A		notes about the sample; sample processing, etc
38	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

9002 data points

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