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Results of the Micro-12 Flight Experiment: Effects of Microgravity on Shewanella oneidensis MR-1The Micro-12 flight experiment was launched on SpaceX-15 and completed during berthing on the International Space Station. The goal of this experiment was to understand the effects of spaceflight and microgravity on the physiology of the model exoelectrogen Shewanella oneidensis MR-1. BioServe Fluid Processing Apparatus (FPA) and Group Activation Pack (GAP) hardware systems were used for both flight and ground control tests. Under spaceflight conditions, extracellular electron transfer (EET) rates were found to be significantly increased on insoluble substrates, while biofilm development appeared to be unchanged under the conditions tested; these processes are critical for microbial-assisted bioelectrochemical systems. Additionally, RNAseq analysis, proteomic profiling, and competitive mutant fitness profiling were performed to gain further understanding of microbial physiology under EET-respiring conditions during spaceflight. Overall, the results of the Micro-12 project support the idea that Shewanella oneidensis MR-1, in particular, and exoelectrogens in general could be useful chassis organisms for synthetic biology applications using microbial bioelectrochemical systems. These findings will assist bioengineering and synthetic biology development efforts harnessing the unique capabilities of exoelectrogens for life support and in situ resource utilization.
Document ID
20190033397
Acquisition Source
Ames Research Center
Document Type
Presentation
Authors
Dougherty, Michael (KBRwyle Moffett Field, CA, United States)
Deutschbauer, Adam (Lawrence Berkeley National Laboratory Berkeley, CA, United States)
Ball, Natalie (KBRwyle Moffett Field, CA, United States)
Karouia, Fathi ORCID(KBRwyle Moffett Field, CA, United States)
Price, Morgan ORCID(Lawrence Berkeley National Laboratory Berkeley, CA, United States)
Ray, Jayashree (Lawrence Berkeley National Laboratory Berkeley, CA, United States)
Arkin, Adam ORCID(Lawrence Berkeley National Laboratory Berkeley, CA, United States)
Hogan, John (NASA Ames Research Center Moffett Field, CA, United States)
Date Acquired
December 5, 2019
Publication Date
November 20, 2019
Subject Category
Life Sciences (General)
Report/Patent Number
ARC-E-DAA-TN75761
Meeting Information
Meeting: Annual Meeting of the American Society for Gravitational and Space Research (ASGSR)
Location: Denver, CO
Country: United States
Start Date: November 20, 2019
End Date: November 23, 2019
Sponsors: American Society for Gravitational and Space Research (ASGSR)
Funding Number(s)
CONTRACT_GRANT: 80NSSC18M0064
CONTRACT_GRANT: NNA17BF58
CONTRACT_GRANT: NNA14AB82C
Distribution Limits
Public
Copyright
Public Use Permitted.
Technical Review
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