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Radiation Stability Evaluation of Protein-Based Nanopores for Mars and Europa MissionsExploration of our Solar System has revealed a number of locations that are now habitable or could have supported life in the past. One approach to finding life involves detection of informational polymers like deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) that are definitive biosignatures for life as we know it. Alternatively, structural variants of DNA and RNA, collectively termed xenonucleic acids (XNAs) have been shown in the laboratory to behave similarly. Nanopore-based sequencers differ from traditional sequencing technologies in that they do not explicitly require synthesis of DNA before or during analysis. Because of this, nanopore sequencers have been used for the direct sequencing of RNA, and could be used for the detection and analysis of other charged polymers. Here we describe results of exposing the MinION hardware, flow cells, and key reagents to ionizing radiation at doses relevant to Mars and Europa missions (10 to 3000 silicon-equivalent gray).
Document ID
20190002053
Acquisition Source
Johnson Space Center
Document Type
Preprint (Draft being sent to journal)
Authors
Burton, Aaron S. (NASA Johnson Space Center Houston, TX, United States)
Sutton, Mark A. (Wichita State Univ. Wichita, KS, United States)
Zaikova, Elena (Georgetown Univ. Washington, DC, United States)
Sutton, Ryan E. (Google, Inc. Mountain View, CA, United States)
Brinckerhoff, William B. (Wichita State Univ. Wichita, KS, United States)
Bevilacqua, Julie G. (Georgetown Univ. Washington, DC, United States)
Weng, Margaret M. (Washington Univ. Saint Louis, MO, United States)
Mumma, Michael J. (Wichita State Univ. Wichita, KS, United States)
Johnson, Sarah S. (Georgetown Univ. Washington, DC, United States)
Date Acquired
March 29, 2019
Publication Date
January 1, 2019
Subject Category
Lunar And Planetary Science And Exploration
Report/Patent Number
JSC-E-DAA-TN66686
Distribution Limits
Public
Copyright
Public Use Permitted.

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