Abstract
The yeast Saccharomyces cerevisiae has three genetic loci encoding different alcohol dehydrogenase (ADH) isozymes: ADC1, which encodes the classical fermentative isozyme ADHI1; ADR2, which encodes the glucose-repressed isozyme ADHII2; and ADM, which encodes an ADH isozyme found associated with mitochondria. When yeast are grown on glucose, the ADC1 gene is expressed, and the ADR2 gene repressed1,3,4. Conversely, growth on a non-fermentable carbon source such as ethanol or glycerol results in derepression of ADR2, and repression of ADC1. The ADC1 and ADR2 genes have been cloned and sequenced5–8, and a number of cis-acting mutations identified that cause constitutive expression of ADR29,10, and seem to fall into two classes11. The most abundant class consists of mutants that cannot be fully derepressed, and do not revert to wild type at a detectable level: these are caused by the insertion of a transposable element into the 5′-flanking region of the gene6,12. The second class of mutants do revert to a glucose-repressed phenotype at a detectable frequency, and when grown on non-fermentable carbon sources derepress ADR2 to levels up to five times those found in wild-type cells10. We report here the sequencing of the 5′-flanking regions of two such promoter-up, constitutive ADR2 mutants, in both of which the mutant phenotype is associated with an increase in length of a poly(A)·poly(T) tract 222 base pairs (bp) upstream of the gene.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Ciriacy, M. Mutat. Res. 29, 315–326 (1975).
Ciriacy, M. Molec. gen. Genet. 138, 157–164 (1975).
Lutstorf, U. & Megnet, R. Archs Biochem. Biophys. 126, 933–944 (1968).
Denis, C., Ciriacy, M. & Young, E. T. J. molec. Biol. 148, 355–368 (1981).
Williamson, V. M., Bennetzen, J., Young, E. T., Nasmyth, K. & Hall, B. D. Nature 283, 214–216 (1980).
Williamson, V. M., Young, E. T. & Ciriacy, M. Cell 23, 605–614 (1981).
Bennetzen, J. L. & Hall, B. D. J. biol. Chem. 257, 3018–3025 (1982).
Russell, D. W., Smith, M., Williamson, V. M. & Young, E. T. J. biol. Chem. 258, 2674–2682 (1983).
Ciriacy, M. Molec. gen. Genet. 145, 327–333 (1976).
Ciriacy, M. Molec. gen. Genet. 176, 427–431 (1979).
Ciriacy, M. & Williamson, V. M. Molec. gen. Genet. 182, 159–163 (1981).
Williamson, V. M., Cox, D., Young, E. T., Russell, D. W. & Smith, M. Molec. cell. Biol. 3, 20–31 (1983).
Struhl, K., Stinchcomb, D. T., Scherer, S. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 76, 1035–1039 (1979).
Grunstein, M. & Hogness, D. S. Proc. natn. Acad. Sci. U.S.A. 72, 3961–3965 (1975).
Messing, J., Crea, R. & Seeburg, P. H. Nucleic Acids Res. 9, 309–321 (1981).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
Maxam, A. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).
Beier, D. R. & Young, E. T. Nature 300, 724–728 (1982).
Walmsley, R. M., Szostak, J. W. & Petes, T. D. Nature 302, 84–86 (1983).
Kunkel, G. R. & Martinson, H. G. Nucleic Acids Res. 9, 6869–6888 (1981).
Prunell, A. EMBO J. 1, 173–179 (1982).
Sledziewski, A. & Young, E. T. Proc. natn. Acad. Sci. U.S.A. 79, 253–256 (1982).
Nasmyth, K. A. Cell 30, 567–578 (1982).
Sanger, F. & Coulson, A. R. FEBS Lett. 87, 107–110 (1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Russell, D., Smith, M., Cox, D. et al. DNA sequences of two yeast promoter-up mutants. Nature 304, 652–654 (1983). https://doi.org/10.1038/304652a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/304652a0
This article is cited by
-
Simple repetitive sequences and gene expression
Molecular Biology (2000)
-
Characterization of trans-acting mutations affecting Ty and Ty-mediated transcription in Saccharomyces cerevisiae
Current Genetics (1991)
-
High mobility group chromosomal proteins bind to AT-rich tracts flanking plant genes
Plant Molecular Biology (1991)
-
Transcription of two divergently transcribed yeast genes initiates at a common oligo(dA-dT) tract
Molecular and General Genetics MGG (1990)
-
DNA damage activates transcription and transposition of yeast Ty retrotransposons
Molecular and General Genetics MGG (1989)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.