Abstract
Human tumour necrosis factor has about 30% homology in its amino acid sequence with lymphotoxin, a lymphokine that has similar biological properties. Recombinant tumour necrosis factor can be obtained by expression of its complementary DNA in Escherichia coli and induces the haemorrhagic necrosis of transplanted methylcholanthrene-induced sarcomas in syngeneic mice.
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References
Carswell, E. A. et al. Proc. natn. Acad. Sci. U.S.A. 72, 3666–3670 (1975).
Ruff, M. R. & Gifford, G. E. in Lymphokines Vol. 2 (ed. Pick, E.) 235–275 (Academic, New York, 1981).
Stone-Wolff, D. S. et al. J. exp. Med. 159, 828–843 (1984).
Helson, L., Green, S., Carswell, E. & Old, L. J. Nature 258, 731–732 (1975).
Matthews, N. & Watkins, J. F. Br. J. Cancer 38, 302–309 (1978).
Matthews, N. Immunology 44, 135–142 (1981).
Ruff, M. R. & Gifford, G. E. Infect. Immun. 31, 380–385 (1981).
Green, S. et al. Proc. natn. Acad. Sci. U.S.A. 73, 381–385 (1976).
Hoffman, M. K., Oettgen, H. F., Old, L. J., Mittler, R. S. & Hammerling, U. J. reticuloendothel. Soc. 23, 307–319 (1978).
Männel, D. N., Moore, R. N. & Mergenhagen, S. E. Infect. Immun. 30, 523–530 (1980).
Aggarwal, B. B., Moffat, B. & Harkins, R. N. J. biol. Chem. 259, 686–691 (1984).
Aggarwal, B. B., Henzel, W. J., Moffat, B., Kohr, W. J. & Harkins, R. N. J. biol. Chem. (in the press).
Gray, P. W. et al. Nature 312, 721–724 (1984).
Collins, S. J., Gallo, R. C. & Gallagher, R. E. Nature 270, 347–349 (1977).
Sundstrom, C. & Nilsson, K. Int. J. Cancer 17, 565–577 (1976).
Aggarwal, B. B. et al. J. biol. Chem. (in the press).
Matteucci, M. & Caruthers, M. H. J. Am. chem. Soc. 103, 3185–3190 (1981).
Grantham, R., Gautier, C., Gouy, M., Jacobzone, M. & Mercier, R. Nucleic Acids Res. 9, r43–73 (1981).
Gray, P. W. et al. Nature 295, 503–508 (1982).
Pennica, D. et al. Nature 301, 214–221 (1983).
Anderson, S. & Kingston, I. B. Proc. natn. Acad. Sci. U.S.A. 80, 6838–6842 (1983).
Ullrich, A., Berman, C. H., Dull, T. J., Gray, A. & Lee, J. M. EMBO J. 3, 361–364 (1984).
Derynck, R., Roberts, A. B., Winkler, M. E., Chen, E. Y. & Goeddel, D. V. Cell 38, 287–297 (1984).
Jaye, M. et al. Nucleic Acids Res. 11, 2325–2335 (1983).
Ullrich, A. et al. Nature 309, 418–425 (1984).
Huynh, T. V., Young, R. A. & Davis, R. W. in DNA Cloning Techniques: A Practical Approach (ed. Glover, D.) (IRL, Oxford, in the press).
Proudfoot, N. J. & Brownlee, G. G. Nature 263, 211–214 (1976).
Nissen-Meyers, J. & Hammerstrom, J. Infect. Immun. 38, 67–73 (1982).
Kozak, M. Nature 308, 241–246 (1984).
Blobel, G. et al. Symp. Soc. exp. Biol. 33, 9–36 (1979).
Watson, M. E. E. Nucleic Acids Res. 12, 5145–5164 (1984).
Hunter, E. et al. J. Virol. 46, 920–936 (1983).
Nakanishi, S. et al. Nature 278, 423–427 (1979).
Noda, M. et al. Nature 295, 202–206 (1982).
Gubler, U. et al. Nature 295, 206–208 (1982).
Amara, S. G., Jonas, V., Rosenfeld, M. G., Ong, E. S. & Evans, R. M. Nature 298, 240–244 (1982).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).
Rinderknecht, E., O'Connor, B. H. & Rodriquez, H. J. biol. Chem. 259, 6790–6797 (1984).
Zoller, M. J. & Smith, M. Meth. Enzym. 100, 468–500 (1983).
Kumagai, K., Itoh, K., Hinuma, S. & Tapa, M. J. immun. Meth. 29, 17–25 (1979).
Wetzel, R. et al. Biochemistry 19, 6096–6104 (1980).
Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).
Wood, W. I. et al. Nature 312, 330–337 (1984).
Benton, W. D. & Davis, R. W. Science 196, 180–182 (1977).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
Messing, J. & Vieira, J. Gene 19, 269–276 (1982).
Messing, J., Crea, R. & Seeburg, P. H. Nucleic Acids Res. 9, 309–321 (1981).
Grosjean, H. & Fiers, W. Gene 18, 199–209 (1982).
Shepard, H. M., Yelverton, E. & Goeddel, D. V. DNA 1, 125–131 (1982).
Gray, G. L. et al. Proc. natn. Acad. Sci. U.S.A. 81, 2645–2649 (1984).
Maizels, N. Nature 249, 647 (1974).
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Pennica, D., Nedwin, G., Hayflick, J. et al. Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin. Nature 312, 724–729 (1984). https://doi.org/10.1038/312724a0
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DOI: https://doi.org/10.1038/312724a0
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