Abstract
SINCE the demonstration by Kipnis and Noall1 that insulin stimulates the accumulation of the non-utilizable amino-acid α-amino-isobutyric acid (AIB) by cells of the intact rat diaphragm in vitro, attempts have been made to relate this phenomenon to the action of insulin on protein synthesis. A review of the available evidence led Manchester and Young2 to the conclusion that the action of insulin on accumulation of amino-acid by the diaphragm of the rat is probably limited to non-utilizable amino-acids and that it may represent an effect secondary to a direct stimulation of protein synthesis. In their view, AIB accumulation “can be regarded as filling the gap left by amino-acids used up in the synthesis of extra protein which occurs in the presence of insulin”. The more recent observation3 that insulin increases the transport of a number of naturally occurring amino-acids into muscle cells prompted us to re-examine the possibility that the effect of insulin on AIB accumulation may be a direct action of the hormone on an amino-acid transport system.
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References
Kipnis, D. M., and Noall, M. W., Biochim. Biophys. Acta., 28, 226 (1958).
Manchester, K. L., and Young, F. G., Vitamins and Hormones, 19, 95 (1961).
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Peckham, W. D., and Knobil, E., Biochim. Biophys. Acta, 63, 207 (1962).
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Rabinovitz, M., and Fisher, J. M., J. Biol. Chem., 237, 477 (1962).
Knobil, E., and Roth, P., Proc. Forty fifth Meeting Endocrine Soc., 61 (1963).
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FRITZ, G., KNOBIL, E. In vitro Stimulation by Insulin of α-Amino-isobutyric Acid Transport in the Absence of Protein Synthesis. Nature 200, 682 (1963). https://doi.org/10.1038/200682a0
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DOI: https://doi.org/10.1038/200682a0
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