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A Polysaccharide resembling Alginic Acid from a Pseudomonas Micro-organism

Abstract

A Pseudomonas micro-organism isolated from the sputum of a patient with cystic fibrosis produced unusually large mucoid colonies on agar culture media. This Pseudomonas, identified as No. 8906 by us, tended to revert to a less mucoid form and occasionally demonstrated phage plaques. The mucoid Pseudomonas was grown on Oxoid sensitivity test media. The bacterial growth was scraped from the surface of the media, and a yield of 664 mg of crude polysaccharide was obtained by initial precipitation with ethanol, followed by extraction with 0.01 N potassium hydroxide at pH 10 and repeated precipitation from aqueous solution with 68 per cent ethanol containing 1 per cent sodium acetate.

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LINKER, A., JONES, R. A Polysaccharide resembling Alginic Acid from a Pseudomonas Micro-organism. Nature 204, 187–188 (1964). https://doi.org/10.1038/204187a0

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