Abstract
BECAUSE the lens is an organ which lacks both blood and nerve supplies it has long been felt that it could be cultured with relative ease. Consequently, many attempts have been made in this direction, primarily with material of mammalian origin. Much of this work is reviewed by Schwartz1 and Bito2. Harding, Rothstein and Newman3 have demonstrated that the epithelial cells of rabbit lenses maintained in systems perfused with combinations of various tissue culture media are caused to undergo DNA synthesis and cell division at varying periods of time following isolation, the specific interval depending on the composition of the perfusate. This ‘activation’ of DNA synthesis is of interest inasmuch as large numbers of cells are synchronized in a stage of the nuclear cycle the characteristics of which one would like to be able to elucidate. The fact that the activation is occurring in vitro naturally makes the whole system much more amenable to biochemical dissection than would ordinarily be the case.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Schwartz, B., Amer. Med. Assoc. Arch. Ophthal., 63, 593 (1960).
Bito, L. Z., Ph.D. thesis, Columbia Univ. (1963).
Harding, C. V., Rothstein, H., and Newman, M. B., Exp. Eye Res., 1, 457 (1962).
Harding, C. V., and Srinivasan, B. D., Exp. Cell Res., 25, 326 (1961).
Rothstein, H., and Harding, C. V., Nature, 194, 294 (1962).
Rothstein, H., Weinsieder, A., and Blaiklock, R., Exp. Cell Res., 35, 548 (1964).
Rothstein, H., Reddan, J., and Weinsieder, A., Exp. Cell Res. (in the press).
Morgan, J. F., Morton, H. J., and Parker, R. C., Proc. Soc. Exp. Biol. Med., 73, 1 (1950).
Wolf, K., and Quimby, M. C., Science, 144, 1578 (1964).
Howard, A., Stain Technol., 27, 313 (1952).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
ROTHSTEIN, H., LAUDER, J. & WEINSIEDER, A. In vitro Culture of Amphibian Lenses. Nature 206, 1267–1269 (1965). https://doi.org/10.1038/2061267a0
Issue Date:
DOI: https://doi.org/10.1038/2061267a0
This article is cited by
-
Differentiation of transplanted lens epithelium of larval Xenopus laevis
Rendiconti Lincei (1994)
-
Amphibian organ culture
Experientia (1973)
-
Long-term cell culture of two differentiated cell types from the liver of larval and adult Xenopus laevis
Zeitschrift f�r Zellforschung und mikroskopische Anatomie (1972)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.