Abstract
IGNORANCE of the genetics of the nitrogen-fixing symbiosis between Rhizobium species and leguminous plants is due chiefly to the lack of a good system for genetic mapping in Rhizobium. Although gene transfer by transformation and transduction have been reported in several Rhizobium species1,2, the only linkage map has come from studies of conjugation in a non-nodulating R. lupini strain3 and a genetic analysis of symbiotically defective mutants has not been possible. R. leguminosarum is (particularly suitable for genetic studies since it nodulates the pea (Pisum sativum), a genetically well known legume4,5. Initial studies with many R. leguminosarum wild types failed to demonstrate recombination6 although P group R factors could be transferred between them6,7. These plasmids have wide host ranges8 and have been shown to mediate the transfer of chromosomal genes in Pseudomonas9, Escherichia10 and Acinetobacter11. R68.45 is a derivative of the P group R factor (R68) isolated by Haas and Holloway9 for having much better sex factor activity in P. aeruginosa than R68 and other R factors. We report here that it behaves in a similar manner in nodulating strains of R. leguminosarum and can be used for genetic mapping in this species.
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BERINGER, J., HOPWOOD, D. Chromosomal recombination and mapping in Rhizobium leguminosarum. Nature 264, 291–293 (1976). https://doi.org/10.1038/264291a0
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DOI: https://doi.org/10.1038/264291a0
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