Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

An actin-binding protein from Acanthamoeba regulates actin filament polymerization and interactions

Nature volume 288, pages 455–459 (1980)Cite this article

Abstract

A protein has been purified from Acanthamoeba which, like cytochalasin B, caps the end of actin filaments normally favoured for monomer addition and inhibits the interactions of actin filaments. In addition, this ‘capping’ protein nucleates the polymerization of actin monomers and blocks the annealing of actin filament fragments.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Taylor, D. L. & Condeelis, J. S. Int. Rev. Cytol. 56, 57–144 (1979).

    Article  CAS  Google Scholar 

  2. Carlsson, L., Nystrom, L. E., Sundkvisk, I., Markey, F. & Lindberg, U. J. molec. Biol. 115, 465–483 (1977).

    Article  CAS  Google Scholar 

  3. Reichstein, E. & Korn, E. D. J. biol. Chem. 254, 6174–6179 (1979).

    CAS  PubMed  Google Scholar 

  4. Tilney, L. G. & Cardell, R. R. J. Cell Biol. 47, 408–419 (1970).

    Article  CAS  Google Scholar 

  5. Tilney, L. G. & Kallenbach, N. J. Cell Biol. 81, 608–623 (1979).

    Article  CAS  Google Scholar 

  6. Maruyama, K. et al. J. Biochem. 81, 215–232 (1977).

    Article  CAS  Google Scholar 

  7. Huxley, H. E. J. molec. Biol. 7, 281–308 (1963).

    Article  CAS  Google Scholar 

  8. Woodrum, D. T., Rich, S. & Pollard, T. D. J. Cell Biol. 67, 231–237 (1975).

    Article  CAS  Google Scholar 

  9. Heizmann, C. & Hauptle, M. Eur. J. Biochem. 80, 443–451 (1977).

    Article  CAS  Google Scholar 

  10. Hatano, S. et al. in Cell Motility: Molecules and Organization (eds Hatano, S., Ishikawa, H. & Sato, H.) 87–104 (University of Tokyo Press, 1979).

    Google Scholar 

  11. Hinssen, H. in Cell Motility: Molecules and Organization (eds Hatano, S., Ishikawa, H. & Sato, H) 59–85 (University of Tokyo Press 1979).

    Google Scholar 

  12. Yin, H. L. & Stossel, T. P. Nature 281, 583–586 (1979).

    Article  ADS  CAS  Google Scholar 

  13. Hartwig, J. H. & Stossel, T. P. J. Cell Biol. 71, 295–302 (1976).

    Article  CAS  Google Scholar 

  14. Maruta, H. & Korn, E. D. J. biol. Chem. 252, 399–402 (1977).

    CAS  PubMed  Google Scholar 

  15. Bryan, J. & Kane, R. E. J. molec. Biol. 125, 207–224 (1978).

    Article  CAS  Google Scholar 

  16. MacLean-Fletcher, S. & Pollard, T. D. J. Cell Biol. 85, 414–428 (1980).

    Article  CAS  Google Scholar 

  17. Hartwig, J. H. & Stossel, T. P. J. molec. Biol. 134, 539–553 (1979).

    Article  CAS  Google Scholar 

  18. MacLean-Fletcher, S. & Pollard, T. D. Cell 20, 329–341 (1980).

    Article  CAS  Google Scholar 

  19. Isenberg, G. & Wohlfarth-Bottermann, K. E. Cell Tissue Res. 173, 495–528 (1976).

    Article  CAS  Google Scholar 

  20. Maruyama, K., Kaibara, M. & Fukada, E. Biochim. biophys. Acta 271, 20–29 (1974).

    Article  Google Scholar 

  21. MacLean-Fletcher, S. & Pollard, T. D. Biochem. biophys. Res. Commun. 96, 18–27 (1980).

    Article  CAS  Google Scholar 

  22. Pollard, T. D., Stafford, W. F. & Porter, M. E. J. biol. Chem. 253, 4798–4808 (1978).

    CAS  Google Scholar 

  23. Ackers, G. K. J. biol. Chem. 242, 3237–3238 (1967).

    CAS  Google Scholar 

  24. Oosawa, F. & Asakura, S. Thermodynamics of the Polymerization of Protein (Academic, New York, 1975).

    Google Scholar 

  25. Pollard, T. D. & Mooseker, M. J. Cell Biol. (submitted).

  26. Moore, P. B., Huxley, H. E. & DeRosier, D. J. J. molec. Biol. 50, 279–295 (1970).

    Article  CAS  Google Scholar 

  27. Pollard, T. D., Levy, J., Isenberg, G. & Aebi, U. J. Cell. Biol. 87, 223a (1980).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cell Biology and Anatomy, The John Hopkins University School of Medicine, Baltimore, Maryland, 21205

    Gerhard Isenberg, Ueli Aebi & Thomas D. Pollard

Authors
  1. Gerhard Isenberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ueli Aebi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Thomas D. Pollard
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Isenberg, G., Aebi, U. & Pollard, T. An actin-binding protein from Acanthamoeba regulates actin filament polymerization and interactions. Nature 288, 455–459 (1980). https://doi.org/10.1038/288455a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/288455a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing