Abstract
The nature of the interactions between cells of the immune system has been shown to be directly related to a group of antigens present on the surface of B lymphocytes1. These antigens are under genetic control of the HLA-D/DR region of the human major histocompatibility complex (MHC) located on chromosome 62. DR antigens (the products of the HLA-DR locus within this region), have been defined by serological studies. Recently another antigen, DC-1, has been serologically defined3,4. In the mouse, the I region, analogous to the HLA-D/DR region in man, has been subdivided into at least five subregions (A, B, J, E and C) and specific functions and cell populations associated with each of the subregions5. Although in man the HLA-D/DR region cannot at present be subdivided, limited NH2-terminal amino acid sequence data have shown that the DR molecule is homologous to the I-E molecule of the mouse6,7. Furthermore, evidence that DC-1 is not homologous to I-E (and might therefore be homologous to I-A) has been reported8. We now report that, using immunoadsorbents prepared with monoclonal antibodies specific for DR or DC-1 antigens, these two human alloantigens were purified from a human lymphoblastoid cell line (LB) and the chains were separated. The NH2-terminal amino acid sequence of the heavy chain of DC-1 antigen shows great structural homology with the murine I-A molecules, thus providing direct evidence of homology of DC-1 to murine I-A.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Strominger, J. L. et al. in The Role of the Major Histocompatibility Complex in Immunobiology (ed. Dorf, M. E.) 115–171 (Garland, New York, 1981).
McKusick, V. A. & Ruddle, F. H. Science 196, 390 (1977).
Tosi, R., Tanigaski, N., Centis, N., Ferrara, G. B. & Pressman, D. J. exp. Med. 148, 1592–1611 (1978).
Shackelford, D. A., Mann, D. L., van Rood, J. J., Ferrara, G. B. & Strominger, J. L. Proc. natn. Acad. Sci. U.S.A. 78, 4566–4570 (1981).
Shreffler, D. C., David, C. S., Cullen, S. E., Frelinger, J. A. & Neiderhuber, J. E. Cold Spring Harb. Symp. quant. Biol. 41, 477 (1976).
McMillan, J., Cecka, J. M., Murphy, D. B., McDevitt, H. O. & Hood, L. Proc. natn. Acad. Sci. U.S.A. 74, 5135–5139 (1977).
Allison, J. P. et al. Proc. natn. Acad. Sci. U.S.A. 75, 3953–3956 (1978).
Corte, G. et al. Nature 292, 357–360 (1981).
Batchelor, J. R. & Morris, P. J. in Histocompatibility Testing 1977, 205–258 (Munsksgaard, Copenhagen, 1978).
Terasaki, P. I. in Histocompatibility Testing 1980, 18–20 (UCLA Press, Los Angeles, 1980).
Duquesnoy, R., Marrari, M. & Vieira, J. in Histocompatibility Testing 1980, 861–863 (UCLA Press, Los Angeles, 1980).
Shackelford, D. A., Kaufman, J. F., Korman, A. J. & Strominger, J. L. Immun. Rev. 66, 133–187 (1982).
Lampson, L. A. & Levy, R. J. Immun. 125, 293–299 (1980).
Brodsky, F. M., Parham, P. & Bodmer, W. F. Tissue Antigens 16, 30–48 (1980).
Silver, J., Cecka, J. M., McMillan, M. & Hood, L. Cold Spring Harb. Symp. quant. Biol. 41, 369 (1977).
Goyert, S. M. & Silver, J. Nature 294, 266–268 (1981).
Auffray, C., Korman, A. J., Roux-Dosseto, M., Bono, R. & Strominger, J. L. Proc. natn. Acad. Sci. U.S.A. 79 (in the press).
Goyert, S. M., Shively, J. E. & Silver, J. J. exp. Med. 156, 550–566 (1982).
March, S. C., Parikh, I. & Cuatrecasas, P. Analyt. Biochem. 60, 149–152 (1974).
Kowal, R. & Parsons, R. Analyt. Biochem. 102, 72–76 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bono, M., Strominger, J. Direct evidence of homology between human DC-1 antigen and murine I-A molecules. Nature 299, 836–838 (1982). https://doi.org/10.1038/299836a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/299836a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
