Abstract
No systematic attempts at culturing this liver fluke in vitro have yet been made. The survival times in published accounts are very short, and physiological work on such short-lived organisms is highly suspect. Ordinary Ringer solutions were used, and the flukes lived from 5 to 12 hr.1,2,3,4.
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References
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STEPHENSON, W. Survival of Fasciola hepatica L. in vitro. Nature 155, 240–241 (1945). https://doi.org/10.1038/155240b0
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DOI: https://doi.org/10.1038/155240b0
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