Abstract
AFTER the chemical synthesis of thiamine phosphoric esters was achieved1–4, reports were published on their cocarboxylasic activity3–7. Investigations have shown that monophosphothiamine has no cocarboxylasic activity, but different values have been found for the activity of triphosphothiamine. The discrepancies may be accounted for by the facts that: (a) chemical synthesis provides mixtures of three esters (mono-, di- and tri-phosphoric) which are very difficult to separate; (b) diphosphothiamine is very active; and therefore quantities as small as 0.1 γ/ml. occurring as impurities with the other esters invalidate the results; (c) washed yeast, which has usually been employed as apoenzyme, makes it difficult to reproduce results.
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DE LA FUENTE, G., DÍAZ-CADAVIECO, R. Cocarboxylasic Activity of Thiamine Phosphoric Esters. Nature 174, 1014 (1954). https://doi.org/10.1038/1741014a0
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DOI: https://doi.org/10.1038/1741014a0
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