Abstract
WHEN leaves of broad bean (Vicia faba L.) are sprayed with suspensions of spores of Botrytis fabae Sardiña they develop chocolate-coloured lesions1. By rubbing suitably diluted spore suspensions over the upper surfaces of leaves of the variety Seville Longpod with the forefinger, and then keeping the inoculated plants at about 20° C. in a saturated atmosphere, we find that the discrete lesions are uniformly distributed and, after 24 hr., can be easily counted. The lesions provide an assay method comparable with counting the local lesions that are produced in plants by many viruses, and we have used them to measure how ultra-violet radiation affects the infective capacity of the spores.
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References
Sardiña, J. R., Mem. Soc. Esp. Hist. Nat., 15, 291 (1929).
Kelner, A., J. Bact., 58, 511 (1949).
Dulbecco, R., J. Bact., 59, 329 (1950).
Bawden, F. C., and Kleczkowski, A., J. Gen. Microbiol., 8, 145 (1953).
Kleczkowski, A., Ann. App. Biol., 36, 139 (1949).
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LAST, F., BUXTON, E. Photo-reactivation of Botrytis fabae Sardiña measured by a Local-lesion Technique. Nature 176, 655 (1955). https://doi.org/10.1038/176655a0
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DOI: https://doi.org/10.1038/176655a0
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