Abstract
THE usual method for staining bacterial and yeast spores requires three operations: the staining of both spores and vegetative cells by applying heat to the slide bearing the dye; the removal of stain from the vegetative cells with a decolorizing agent; and counterstaining the vegetative cells with a dye of a different colour. The same treatment is used to differentiate acid-fast bacteria from others.
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GRAY, P. Staining of Bacteria and Certain Fungi. Nature 147, 329 (1941). https://doi.org/10.1038/147329b0
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DOI: https://doi.org/10.1038/147329b0
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